Applications

Lab on a Bead's concept for antibody purification using LOABeads magnetic beads and LOABeads MagSep separators can be used in a wide variety of applications. From small scale screening of several samples in parallel to purification of antibodies from tens or hundreds of milliliters sample volumes. The beads are also perfect for use in applications where multiple ligands are bound subsequently to the beads, for example in immunoprecipitation and various biosensors

Application notes

LOABeads PrtA30

Parallel purification of scFv antibody fragments using LOABeads™ PrtA30 (PDF)
In this study, LOABeads PrtA30 were used together with a KingFisher™ Flex robot and the LOABeads MagSep separators, to purify expressed scFv antibody fragments from 500 ml bacterial cultures. A high purity was achieved for the four different scFv proteins that were purified and they were shown to be active in an ELISA test.
Product: LOABeads PrtA30 (Prod. No. 1100) Published: October 2016
Time study of parallel mAb purification from clarified CHO-cell broth at 1ml LOABeads™ PrtA30 scale (PDF)
This application not compares the LOABeads system to instrument operated column chromatography. Monoclonal antibodies were purified from four 200 ml CHO-cell culture samples using 1 ml LOABeads PrtA30 for each and the LOABeads MagSep separators. At this scale, the four antibody samples were processed faster than an estimated purification of one single sample on a column chromatography instrument.
Product: LOABeads PrtA30 (Prod. No. 1100) Published: March 2017

LOABeads PrtA60

A comperative test chromatography vs LOABbeads PrtA60 (PDF)
In this study antibody purification using LOABeads PrtA60 was compared to lab-scale column chromatography, with regards to yield, concentration and purity.
Product: LOABeads PrtA60 (Prod. No. 1200) Published: March 2016
Immunoprecipitation efficiency for short time pulldowns using LOABeads™ PrtA60 (PDF)
The recovery efficiency for immunoprecipitation of human ferritin using LOABeads PrtA60 was measured with a ruthenium based immunoassay, showing that the recovery was very high.

As an alternative, LOABeads IP30 could also be used in this type of immunoprecipitation experiment and be expected to perform equally well.

Product: LOABeads PrtA60 (Prod. No. 1200) Published: July 2016

LOABeads AffiActive10

Immuno-affinity purification of IgG (PDF)
In this study LOABeads AffiActive10 was used to purify anti-rabbit IgG produced in goat antiserum, resulting in high purity antibody.
Product: LOABeads AffiActive10 (Prod. No. 1300) Published: October 2015
Affinity purification of disease biomarkers from human serum (PDF)
In this study LOABeads AffiActive10 was used to isolate autoantibodies, which are biomarkers of Multiple Sclerosis, from patient serum.
Product: LOABeads AffiActive10 (Prod. No. 1300) Published: December 2015

Publications

Label-free bead-based metallothionein electrochemical immunosensor
LOABeads PrtA60 was coated with chicken IgY which specifically recognized metallothionein. The coating was obtained via rabbit IgG raised against chicken IgY, which was bound directly to the beads. Using the Brdicka reaction in a microfluidic 3D-printed chip, it was possible to detect metallothionein in patient samples.
Nejdl, L., Nguyen, H. V., Richtera, L., Krizkova, S., Guran, R., Masarik, M., Hynek, D., Heger Z., Lundberg, K., Eriksson, K., Adam, V., Kizek, R., Electrophoresis 2015, 36, 1894–1904
Product: LOABeads PrtA60 (Prod. No. 1200) Published: August 2015
Highly specific and rapid immuno-fluorescent visualization and detection of E. coli O104:H4 with protein-A coated magnetic beads based LST-MUG assay
LOABeads PrtA60 was coated with an antibody recognizing E. coli O104:H4 and the beads were then bound to whole E. coli. By letting the E. coli with bound magnetic beads grow in LST-MUG media, it was possible to detect low concentrations of E. coli using fluorescence.
Barizuddin, S., Balakrishnan,B., Stringer, R. C., Dweik, M., J. Microbiol. Methods 2015, 115, 27-33
Product: LOABeads PrtA60 (Prod. No. 1200) Published: August 2015
Analysis of serum transthyretin by on-line immunoaffinity solid-phase extraction capillary electrophoresis mass spectrometry using magnetic beads
LOABeads PrtA60 and AffiActive10 was used for immunoprecipitation of transthyretin from serum samples in an on-line immonprecipitation method. In comparison with Protein A magnetic beads from other manufacturers, LOABeads PrtA60 had higher recovery and better repeatability. LOABeads AffiActive10 had the advantage that the antibody did not elute and interfere with detection and was therefore preferred.
Peró-Gascón, R., Pont, L., Benavente, F., Barbosa, J., Sanz-Nebot, V., Electrophoresis 2016, 37, 1220-1231
Product: LOABeads PrtA60, LOABeads AffiActive0 (Prod. No. 1200 and 1300) Published: March 2016