Lab on a Bead’s unique patented coupling chemistry enables fast and efficient covalent conjugation of amino (-NH2) and thiol (-SH) containing ligands, such as proteins and peptides, to surfaces. All steps in the coupling are performed at physiological conditions and at room temperature with high yields and short reaction times. The use of water-based media eliminates the use of organic solvent and no hazardous reagents are needed. This makes Lab on a Bead’s coupling chemistry a more ecofriendly alternative compared to most of the traditionally used coupling techniques, such as the CNBr and NHS methods.
The technology is used in the production of magnetic beads and is available for sale with the LOABeads AffiActive10 beads. Affinity, IEX and hydrophobic ligands can be introduced, making a wide variety of bioseparations possible using magnetic beads.