LOABeads AffiAmino

Activated magnetic beads for coupling of amino containing ligands.

LOABeads AffiAmino are surface activated magnetic agarose beads employing Lab on a Bead’s patented coupling chemistry. Any amino containing ligand, e.g. proteins and antibodies can be covalently bound to the beads using a simple process. All steps are performed at physiological conditions and room temperature, without using any hazardous reagents or organic solvents. After conjugation of the ligand, the magnetic beads can be used for affinity purification and immunoprecipitation.

The binding capacity of LOABeads AffiAmino is 0.5–1 mg of human IgG per ml bead suspension (5–10 mg per ml settled beads), 0.5–0.75 mg Protein A per ml bead suspension (5–7.5 mg per ml settled beads) and similar amounts of other proteins. The bond is very stable (6 ppm leakage of Protein A) and the beads can be stored with bound ligand.

The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers minimal unspecific binding and makes the beads non-adherent, letting the beads be easily dispersed and resuspended without the use of detergents.

The purification setup can easily be scaled up or down to match antibody concentration and sample volumes. LOABeads AffiAmino work excellently for separations from microliter to liter scales using suitable magnetic separators, such as the LOABeads MagSep series.

Material supplied

  • LOABeads AffiAmino supplied as a 10% bead suspension in PBS with 20% ethanol.
  • Activation buffer
  • Neodymium cube magnet (12 mm) suitable for separations in 0.5-5 ml volumes.

Product data

Product name LOABeads™ AffiAmino
Product number 1003
Particle size 45–165 µm
Coupling to Primary amino groups
Type magnetization Super-paramagnetic
Matrix 4% agarose
Product form 10% bead suspension in PBS with 20% ethanol
Coupling capacity* 0.5–1 mg IgG/ml bead suspension

5–10 mg IgG/ml settled beads

Binding buffer PBS with 0.1% tween
Storage conditions +2 to +8°C in PBS with 20% ethanol
Stability information** 12 months

* 90% coupling of rabbit IgG (1 mg/ml) after 60 min incubation.
** Data of product stability is continuously updated based on ongoing stability studies.

Ordering Information

Product Quantity Product no.
LOABeads AffiAmino 2x1 ml 10% beads 1003-2ml
LOABeads AffiAmino 5x1 ml 10% beads 1003-5m
LOABeads AffiAmino 10 ml 10% beads 1003-10ml