LOABeads AffiAmino are surface activated magnetic agarose beads employing Lab on a Bead’s patented coupling chemistry. Any amino containing ligand, e.g. proteins and antibodies can be covalently bound to the beads using a simple process. All steps are performed at physiological conditions and room temperature, without using any hazardous reagents or organic solvents. After conjugation of the ligand, the magnetic beads can be used for affinity purification and immunoprecipitation.
The binding capacity of LOABeads AffiAmino is 0.5–1 mg of human IgG per ml bead suspension (5–10 mg per ml settled beads), 0.5–0.75 mg Protein A per ml bead suspension (5–7.5 mg per ml settled beads) and similar amounts of other proteins. The bond is very stable (6 ppm leakage of Protein A) and the beads can be stored with bound ligand.
The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers minimal unspecific binding and makes the beads non-adherent, letting the beads be easily dispersed and resuspended without the use of detergents.
The purification setup can easily be scaled up or down to match antibody concentration and sample volumes. LOABeads AffiAmino work excellently for separations from microliter to liter scales using suitable magnetic separators, such as the LOABeads MagSep series.
|Product name||LOABeads™ AffiAmino|
|Particle size||45–165 µm|
|Coupling to||Primary amino groups|
|Product form||10% bead suspension in PBS with 20% ethanol|
|Coupling capacity*||0.5–1 mg IgG/ml bead suspension
5–10 mg IgG/ml settled beads
|Binding buffer||PBS with 0.1% tween|
|Storage conditions||+2 to +8°C in PBS with 20% ethanol|
|Stability information**||12 months|
* 90% coupling of rabbit IgG (1 mg/ml) after 60 min incubation.
** Data of product stability is continuously updated based on ongoing stability studies.
|LOABeads AffiAmino||2x1 ml 10% beads||1003-2ml|
|LOABeads AffiAmino||5x1 ml 10% beads||1003-5m|
|LOABeads AffiAmino||10 ml 10% beads||1003-10ml|