LOABeads MabBind A

Magnetic agarose beads optimized for efficient purification of IgG from cell cultures.

LOABeads MabBind A are protein A coupled magnetic agarose beads specifically developed for the purification of monoclonal IgG antibodies from cell culture harvests, such as from hybridoma and CHO cell cultures. The high specificity and high capacity of LOABeads MabBind A ensures that a very pure antibody is obtained with a high yield, even when antibody capture is performed directly in the cell culture medium.

The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers negligible unspecific binding and makes the beads non-adherent. This lets them be easily dispersed and resuspended without the use of detergents.

LOABeads MabBind A are ideal to use in many applications of magnetic bead affinity chromatography (MBAC). For example in lab-scale purification of antibodies to be used in R&D and diagnostics, and for characterizations of candidate biopharmaceutical IgG antibodies. The quantity of beads can readily be scaled up or down and regardless of scale, the same conditions will provide equal yield and purity.

For analytical and small-scale preparative work, as well as method scouting, samples can be handled in standard 15 and 50 ml centrifuge tubes using the LOABeads Magsep 15/50 separator. In the LOABeads MagSep 500 unit, 30 ml of settled beads in a 500 ml sample can be handled, giving a maximum adsorbent capacity of 900 mg IgG.

Material Supplied

  • LOABeads MabBind A supplied as a 10% bead suspension in PBS with 20% ethanol.
    10 ml of 10% bead suspension contains 1 ml magnetic beads.
  • Neodymium cube magnet (12 mm) suitable for separations in 0.5-5 ml volumes.

Product Data

Product name LOABeads™ MabBind A
Product number 1004
Particle size 45–165 µm
Ligand Recombinant protein A
Type magnetization Super-paramagnetic
Matrix 4% agarose
Product form 10% bead suspension in PBS with 20% ethanol
Binding capacity* 3 mg rabbit IgG/ml bead suspension
Binding conditions Directly in cell culture media, pH 6–8
Elution buffer 60 mM citrate pH 3.0
Storage conditions +2 to +8°C in PBS with 20% ethanol
Stability information** 12 months
Regeneration Can be reused multiple times
* Binding capacity was determined by incubating 500 μl 10% LOABeads MabBind A (50 μl beads) with rabbit IgG (~2 mg/ml in 1 ml PBS) for 60 minutes at room temperature. Binding capacity is obtained with a yield of 90% or more under the conditions specified above.
** Data of product stability is continuously updated based on ongoing stability studies.

 

Ordering Information

Product Quantity Product no.
LOABeads MabBind A 1 ml beads (in suspension) 1004-1ml
LOABeads MabBind A 5 ml beads (in suspension) 1004-5ml
LOABeads MabBind A 25 ml beads (in suspension) 1004-25ml