LOABeads Protein A have a binding capacity 60 mg human or rabbit IgG per ml settled magnetic beads. The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers minimal unspecific binding and makes the beads non-adherent, letting the beads be easily dispersed and resuspended without the use of detergents.
The purification setup can easily be scaled up or down to match antibody concentration and sample volumes. LOABeads Protein A work excellently for separations from microliter to liter scales using suitable magnetic separators, such as the LOABeads MagSep series.
LOABeads Protein A are intended for purification of antibodies from serum and ascites fluid samples, IgG serum depletion, and immunoprecipitation. The product is for research use only and not intended for human use or for use in diagnostic applications.
|Product name||LOABeads™ Protein A|
|Particle size||45–165 µm|
|Ligand||Recombinant protein A|
|Product form||10% bead suspension in PBS with 20% ethanol|
|Binding capacity*||4 mg rabbit IgG/ml bead suspension|
|Max. binding capacity**||6 mg human IgG/ml bead suspension|
|Elution buffer||60 mM citrate pH 3.0|
|Storage conditions||+2 to +8°C in PBS with 20% ethanol|
|Stability information***||24 months|
|Regeneration||Can be reused multiple times|
* 90% binding of rabbit IgG (2 mg/ml) after 60 min reaction.
** Determined in an overloading test at 5 mg IgG/ml after 60 min reaction.
*** Data of product stability is continuously updated based on ongoing stability studies.
|LOABeads Protein A||2x1 ml 10% beads||1001-2ml|
|LOABeads Protein A||5x1 ml 10% beads||1001-5m|
|LOABeads Protein A||10 ml 10% beads||1001-10ml|
I would definitely recommend LOABeads Protein A to others – you can obtain your antibody with satisfactory yield and purity within a short time.
Yes, I would recommend LOABeads Protein A to other researchers. It can be perfomed using few supplies and reagents.
LOABeads Protein A is the fastest protocol I used in the lab to purify antibodies. Similar performance were observed for both affinity chromatography and magnetic beads methods in terms of obtained antibody quantities.