LOABeads PrtA30

Protein A magnetic beads optimized for efficient purification of IgG from cell cultures.

LOABeads PrtA30 are protein A coupled magnetic agarose beads specifically developed for the purification of monoclonal IgG antibodies from cell culture harvests, such as from hybridoma and CHO cell cultures. The high specificity and high capacity of LOABeads PrtA30 ensures that a very pure antibody is obtained with a high yield, even when antibody capture is performed directly in the cell culture medium.

The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers negligible unspecific binding and makes the beads non-adherent. This lets them be easily dispersed and resuspended without the use of detergents.

LOABeads PrtA30 are ideal to use in many applications of magnetic bead affinity chromatography (MBAC). For example in lab-scale purification of antibodies to be used in R&D and diagnostics, and for characterizations of candidate biopharmaceutical IgG antibodies. The quantity of beads can readily be scaled up or down and regardless of scale, the same conditions will provide equal yield and purity.

For analytical and small-scale preparative work, as well as method scouting, samples can be handled in standard 15 and 50 ml centrifuge tubes using the LOABeads Magsep50 separator. In the LOABeads MagSep500 unit, 30 ml of settled beads in a 500 ml sample can be handled, giving a maximum adsorbent capacity of 900 mg IgG.

Material Supplied

  • LOABeads PrtA30 supplied as a 10% bead suspension in PBS with 20% ethanol.
    10 ml of 10% bead suspension contains 1 ml magnetic beads.

Product Data

Product name LOABeads™ PrtA30
Product number 1100
Particle size 45–165 µm
Ligand Recombinant protein A
Type magnetization Super-paramagnetic
Matrix 4% agarose
Product form 10% bead suspension in PBS with 20% ethanol
Binding capacity* 30 mg rabbit IgG/ml settled beads
Binding conditions Directly in cell culture media, pH 6–8
Elution buffer 60 mM citrate, pH 3.0
100 mM glycine, pH 2.8
Storage conditions +2 to +8°C in PBS with 20% ethanol
Stability information** 18 months
Regeneration Can be reused multiple times
* Binding capacity was determined by incubating 50 μl LOABeads PrtA30 with rabbit IgG (~2 mg/ml in 1 ml PBS) for 60 minutes at room temperature. Binding capacity is obtained with a yield of 90% or more under the conditions specified above.
** Data of product stability is continuously updated based on ongoing stability studies.

 

Ordering Information

Product Quantity Product no.
LOABeads PrtA30 5 ml beads 1101
LOABeads PrtA30 10 ml beads 1102
LOABeads PrtA30 25 ml beads 1103