LOABeads PrtA60 have a binding capacity 60 mg human or rabbit IgG per ml settled magnetic beads. The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers minimal unspecific binding and makes the beads non-adherent, letting the beads be easily dispersed and resuspended without the use of detergents.
The purification setup can easily be scaled up or down to match antibody concentration and sample volumes. LOABeads PrtA60 work excellently for separations from microliter to liter scales using suitable magnetic separators, such as the LOABeads MagSep series.
LOABeads PrtA60 are intended for purification of antibodies from serum and ascites fluid samples and for IgG serum depletion. The product is for research use only and not intended for human use or for use in diagnostic applications.
|Product name||LOABeads™ PrtA60|
|Particle size||45–165 µm|
|Ligand||Recombinant protein A|
|Product form||10% bead suspension in PBS with 20% ethanol|
|Binding capacity*||40 mg rabbit IgG/ml settled beads|
|Max. binding capacity**||60 mg human IgG/ml settles beads|
|Elution buffer||60 mM citrate pH 3.0|
|Storage conditions||+2 to +8°C in PBS with 20% ethanol|
|Stability information***||24 months|
|Regeneration||Can be reused multiple times|
* 90% binding of rabbit IgG (2 mg/ml) after 60 min reaction.
** Determined in an overloading test at 5 mg IgG/ml after 60 min reaction.
*** Data of product stability is continuously updated based on ongoing stability studies.
|LOABeads PrtA60||5 ml beads||1201|
|LOABeads PrtA60||10 beads||1202|
|LOABeads PrtA60||25 ml beads||1203|
I would definitely recommend LOABeads PrtA60 to others – you can obtain your antibody with satisfactory yield and purity within a short time.
Yes, I would recommend LOABeads PrtA60 to other researchers. It can be perfomed using few supplies and reagents.
LOABeads PrtA60 is the fastest protocol I used in the lab to purify antibodies. Similar performance were observed for both affinity chromatography and magnetic beads methods in terms of obtained antibody quantities.