LOABeads PrtA60

High capacity protein A magnetic agarose beads provide rapid and simple purification of antibodies from serum and ascites.

LOABeads PrtA60 have a binding capacity 60 mg human or rabbit IgG per ml settled magnetic beads. The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers minimal unspecific binding and makes the beads non-adherent, letting the beads be easily dispersed and resuspended without the use of detergents.

The purification setup can easily be scaled up or down to match antibody concentration and sample volumes. LOABeads PrtA60 work excellently for separations from microliter to liter scales using suitable magnetic separators, such as the LOABeads MagSep series.

LOABeads PrtA60 are intended for purification of antibodies from serum and ascites fluid samples and for IgG serum depletion. The product is for research use only and not intended for human use or for use in diagnostic applications.

Material Supplied

  • LOABeads PrtA60 supplied as a 10% bead suspension in PBS with 20% ethanol.
    1 ml of 10% bead suspension contains 100 µl magnetic beads.

Product Data

Product name LOABeads™ PrtA60
Product number 1200
Particle size 45–165 µm
Ligand Recombinant protein A
Type magnetization Super-paramagnetic
Matrix 4% agarose
Product form 10% bead suspension in PBS with 20% ethanol
Binding capacity* 40 mg rabbit IgG/ml settled beads
Max. binding capacity** 60 mg human IgG/ml settles beads
Binding buffer PBS
Elution buffer 60 mM citrate pH 3.0
Storage conditions +2 to +8°C in PBS with 20% ethanol
Stability information*** 24 months
Regeneration Can be reused multiple times

* 90% binding of rabbit IgG (2 mg/ml) after 60 min reaction.
** Determined in an overloading test at 5 mg IgG/ml after 60 min reaction.
*** Data of product stability is continuously updated based on ongoing stability studies.

Ordering Information

Product Quantity Product no.
LOABeads PrtA60 5 ml beads 1201
LOABeads PrtA60 10 beads 1202
LOABeads PrtA60 25 ml beads 1203

Yes, I would recommend LOABeads PrtA60 to other researchers. It can be perfomed using few supplies and reagents.
Marcelo Mendonça, Federal Rural University of Pernambuco, Brazil
LOABeads PrtA60 is the fastest protocol I used in the lab to purify antibodies. Similar performance were observed for both affinity chromatography and magnetic beads methods in terms of obtained antibody quantities.
Feliciana Real, University of Florence, Italy