LOABeads™ SerAscA
High capacity protein A magnetic agarose beads provide rapid and simple purification of antibodies from serum and ascites.LOABeads SerAscA have a binding capacity 60 mg human or rabbit IgG per ml settled magnetic beads. The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers minimal unspecific binding and makes the beads non-adherent, letting the beads be easily dispersed and resuspended without the use of detergents.
The purification setup can easily be scaled up or down to match antibody concentration and sample volumes. LOABeads SerAscA work excellently for separations from microliter to liter scales using suitable magnetic separators, such as the LOABeads MagSep series.
LOABeads SerAscA are intended for purification of antibodies from serum and ascites fluid samples and for IgG serum depletion. The product is for research use only and not intended for human use or for use in diagnostic applications.
Material Supplied
- LOABeads SerAscA supplied as a 10% bead suspension in PBS with 20% ethanol.
1 ml of 10% bead suspension contains 100 µl magnetic beads.
Product Data
| Product name | LOABeads™ SerAscA |
| Product number | 1200 |
| Particle size | 45–165 µm |
| Ligand | Recombinant protein A |
| Type magnetization | Super-paramagnetic |
| Matrix | 4% agarose |
| Product form | 10% bead suspension in PBS with 20% ethanol |
| Binding capacity* | 40 mg rabbit IgG/ml settled beads |
| Max. binding capacity** | 60 mg human IgG/ml settles beads |
| Binding buffer | PBS |
| Elution buffer | 60 mM citrate pH 3.0 |
| Storage conditions | +2 to +8°C in PBS with 20% ethanol |
| Stability information*** | 24 months |
| Regeneration | Can be reused multiple times |
* 90% binding of rabbit IgG (2 mg/ml) after 60 min reaction.
** Determined in an overloading test at 5 mg IgG/ml after 60 min reaction.
*** Data of product stability is continuously updated based on ongoing stability studies.
Ordering Information
| Product | Quantity | Product no. |
| LOABeads SerAscA | 1 ml beads | 1201 |
| LOABeads SerAscA | 5 beads | 1202 |
| LOABeads SerAscA | 25 ml beads | 1203 |
Documentation
Product Manual
Technical Datasheet
Material Safety Datasheet
Information Flyer
Applications
I would definitely recommend LOABeads SerAscA to others – you can obtain your antibody with satisfactory yield and purity within a short time.
Yes, I would recommend LOABeads SerAscA to other researchers. It can be perfomed using few supplies and reagents.
LOABeads SerAscA is the fastest protocol I used in the lab to purify antibodies. Similar performance were observed for both affinity chromatography and magnetic beads methods in terms of obtained antibody quantities.