Analysis of serum transthyretin using immunoaffinity method based on magnetic beads and capillary electrophoresis

Analysis of serum transthyretin by on-line immunoaffinity solid-phase extraction capillary electrophoresis mass spectrometry using magnetic beads
LOABeads PrtA60 and AffiAmino10 was used for immunoprecipitation of transthyretin from serum samples in an on-line immonprecipitation method. In comparison with Protein A magnetic beads from other manufacturers, LOABeads PrtA60 had higher recovery and better repeatability. LOABeads AffiAmino had the advantage that the antibody did not elute and interfere with detection and was therefore preferred.
Peró-Gascón, R., Pont, L., Benavente, F., Barbosa, J., Sanz-Nebot, V., Electrophoresis 2016, 37, 1220-1231
Product: LOABeads PrtA60, LOABeads AffiAmino10 (Prod. No. 1200 and 1300) Published: March 2016

Immuno-fluorescent detection of E. coli with assay based on protein A magnetic beads

Highly specific and rapid immuno-fluorescent visualization and detection of E. coli O104:H4 with protein-A coated magnetic beads based LST-MUG assay
LOABeads PrtA60 was coated with an antibody recognizing E. coli O104:H4 and the beads were then bound to whole E. coli. By letting the E. coli with bound magnetic beads grow in LST-MUG media, it was possible to detect low concentrations of E. coli using fluorescence.
Barizuddin, S., Balakrishnan,B., Stringer, R. C., Dweik, M., J. Microbiol. Methods 2015, 115, 27-33
Product: LOABeads PrtA60 (Prod. No. 1200) Published: August 2015

Label-free bead-based metallothionein electrochemical immunosensor

Label-free bead-based metallothionein electrochemical immunosensor
LOABeads Protein A was coated with chicken IgY which specifically recognized metallothionein. The coating was obtained via rabbit IgG raised against chicken IgY, which was bound directly to the beads. Using the Brdicka reaction in a microfluidic 3D-printed chip, it was possible to detect metallothionein in patient samples.
Nejdl, L., Nguyen, H. V., Richtera, L., Krizkova, S., Guran, R., Masarik, M., Hynek, D., Heger Z., Lundberg, K., Eriksson, K., Adam, V., Kizek, R., Electrophoresis 2015, 36, 1894–1904
Product: LOABeads Protein A (Prod. No. 1001) Published: August 2015

Immunoprecipitation efficiency for short time pulldowns using LOABeads™ PrtA60

Immunoprecipitation efficiency for short time pulldowns using LOABeads™ Protein A (PDF)
The recovery efficiency for immunoprecipitation of human ferritin using LOABeads PrtA60 was measured with a ruthenium based immunoassay, showing that the recovery was very high.

As an alternative, LOABeads IP30 could also be used in this type of immunoprecipitation experiment and be expected to perform equally well.

Product: LOABeads PrtA60 (Prod. No. 1200) Published: July 2016