LOABeads AlkaliA are super-paramagnetic agarose beads covalently coupled with an alkali-resistant recombinant protein A. The black beads are clearly visible, strongly attracted to external magnets, and easily resuspended. The quantity of beads can readily be scaled up or down to match application.
LOABeads AlkaliA are intended for purification of monoclonal antibodies from cell culture harvests, such as from hybridoma or CHO cells, where sanitization of beads with NaOH is desired. The beads are suitable for separations using appropriate magnetic separators, such as LOABeads MagSep50 (Product No. 2200) and LOABeads MagSep500 (Product No. 2300). The product is intended for research use only.
|Product name||LOABeads™ AlkaliA|
|Particle size||45–165 µm|
|Ligand||Alkali-resistant recombinant protein A|
|Product form||10% bead suspension in PBS with 20% ethanol|
|Binding capacity1||45 mg/ml|
|Static binding capacity||>55 mg/ml settled beads|
|Binding conditions||Directly in cell culture media, pH 6–8|
|Elution conditions2||100 mM citrate pH 2.8
100 mM glycine, pH 2.8
|Storage||+2 to +8°C in PBS containing 20% ethanol|
|Ligand leakage4||10–100 ng/mg IgG (10–100 ppm)|
|Alkali-treatment||Retains 90% activity after 16 hours exposure to 0.1 M NaOH|
|1 Binding capacity at 5 mg IgG/ml sample
2 Some antibodies may require different elution conditions.
3 Data of product stability is continuously updated based on ongoing stability studies.
4 Protein A ligand leakage in the acidic elution fraction with 1 minute contact time at room temperature was determined using a Protein A ELISA kit (#03-96) from Immun System I.M.S AB, Sweden.
|LOABeads™ AlkaliA||1 ml beads5||1501|
|LOABeads™ AlkaliA||5 ml beads||1502|
|LOABeads™ AlkaliA||25 ml beads||1503|