LOABeads™ IP-A
Protein A magnetic beads for immunoprecipitation.LOABeads IP-A are magnetic agarose beads coupled with a recombinant protein A ligand and are intended to be used in immunoprecipitations. The beads have a high binding capacity for different IgG subclasses, which makes the beads more flexible in different applications.
The super-paramagnetic LOABeads IP-A particles are strongly attracted to external magnets allowing efficient separation. The agarose matrix enables minimal nonspecific binding of proteins due to its hydrophilic nature. The black beads are easily observed by the naked eye, making them easy to follow and collect, and they do not aggregate, which facilitates the resuspension.
Material Supplied
- LOABeads IP-A supplied as a 10% bead suspension in PBS with 20% ethanol.
10 ml of 10% bead suspension contains 1 ml magnetic beads.
Product Data
| Product name | LOABeads™ IP-A |
| Product number | 1400 |
| Particle size | 45–165 µm |
| Ligand | Recombinant protein A |
| Type magnetization | Super-paramagnetic |
| Matrix | 4% agarose |
| Product form | 10% bead suspension in PBS with 20% ethanol |
| Binding capacity* | 30 mg rabbit IgG/ml settled beads |
| Binding conditions | Directly in cell culture media, pH 6–8 |
| Elution buffer | 60 mM citrate, pH 3.0 100 mM glycine, pH 2.8 |
| Storage conditions | +2 to +8°C in PBS with 20% ethanol |
| Stability information** | 18 months |
| Regeneration | Cannot be reused |
* Binding capacity was determined by incubating 50 μl LOABeads IP30 with rabbit IgG (~2 mg/ml in 1 ml PBS) for 60 minutes at room temperature. Binding capacity is obtained with a yield of 90% or more under the conditions specified above.
** Data of product stability is continuously updated based on ongoing stability studies.
** Data of product stability is continuously updated based on ongoing stability studies.
Ordering Information
| Product | Quantity | Product no. |
| LOABeads IP30 | 0.5 ml beads | 1401 |
| LOABeads IP30 | 1 ml beads | 1402 |
| LOABeads IP0 | 5 ml beads | 1403 |
