Tips for using LOABeads MagSep separators

Here are some of our best tips for using the magnetic separators in the LOABeads MagSep series smoothly.

LOABeads MagSep Cube

How to hold the LOABeads MagSep Cube
  1. Hold the cube magnet between thumb and two first fingers in your non-dominant hand.
  2. Place the microtube containing magnetic beads in front of the magnet. Hold it, too, between thumb and fingers.
  3. Within seconds the beads will be captured on the side of the tube. Tap the tube lightly on the bench a couple of times, to release minute amount of beads possibly stuck on the opposite wall of the tube.
  4. Pipette off the liquid. Place the tip on the opposite side of the tube compared to the magnet, to disturb the beads as little as possible.
To free up your hand, you can attach the cube magnet to a metallic wire rack for microtubes. Then you simply hold the tube against the magnet to quickly separate the beads, or place the tube in the rack next to the magnet.

LOABeads MagSep 15/50

LOABeads MagSep 15/50

  1. Make sure that the magnetic beads are well suspended.
  2. Place a 15 or 50 ml tube in the correct position.
  3. Allow the magnet to attract the beads, which usually takes 10–15 sec. The process can be observed through the side openings.
  4. Position a pipette in the liquid, away from the beads, and carefully pipette off the solution
If any beads are caught in the bottom of the tube, rotate the tube back and forth in the separator or flush carefully with liquid in the cone using a pipette.

LOABeads MagSep 500

LOABeads MagSep 500

  1. Mix the flask vigorously to resuspend the beads.
  2. Swirl the flask and place it inside the separator while the beads are still moving .
  3. Wait until no beads remain in suspension. It might take several minutes.
  4. Remove the liquid using a pipette coupled to a water suction device or vacuum pump, with a clean safety bottle. Place the pipette tip in the middle of the liquid surface and move it downwards as the liquid is aspirated.
If any beads seem to be caught in the middle of the flask or on the bottom, carefully flush with liquid towards the particles using a pipette. The beads are easier captured by the magnets while moving.